The chromatographically pure compound was obtained. 获得了色谱纯的化合物。
Chromatographically pure chitinase could be obtained by ( NH4) 2SO4 fractional precipitation, anion-exchange chromatography, SDS-PAGE, and a singular and high chromatography peak could be found and two electrophoretic bands could be validated from gel electrophoresis. 几丁质酶通过(NH4)2SO4分级沉淀、阴离子交换柱层析、SDS-PAGE提纯后可以达到层析纯,出现单一并且峰值很高的层析峰,电泳确认两条带,分别为几丁质外切酶和几丁质内切酶。